Unlocking the Hidden Kinome
Michael T. McManus, Ph.D.
Professor, University of California, San Francisco
Vincent and Stella Coates Endowed Chair
Director, UCSF Keck Center for Noncoding RNAs
Core Director, UCSF Sandler Lentiviral RNAi Core
Department of Microbiology and Immunology
UCSF Diabetes Center
The McManus project uses Cas9-based technologies (CRISPRi, CRISPRa, and wtCRISPR) to interrogate GPCR, NR, ion channel, and kinase genes whose perturbation can cooperate or interfere with signal transduction, specifically cell proliferation, cell death, and differentiation. Dr. Michael T. McManus’ work for IDG is applying the CRISPR-CAS technology to unlock the hidden kinome (and will expand to other IDG protein classes) allowing for measuring the connection between genes and pathways by the utility of gene expression, gene activation or gene edition. Goals are to validate these Tdark matter and additional screens for epistatis.
Boettcher M, McManus MT. Choosing the Right Tool for the Job: RNAi, TALEN, or CRISPR. Mol Cell. 2015 May 21;58(4):575-85. doi: 10.1016/j.molcel.2015.04.028. PMCID: PMC4441801, PubMed Abstract: http://www.ncbi.nlm.nih.gov/pubmed/26000843
- Michael McManus' lab: http://mcmanuslab.ucsf.edu/
- Western Coalition of IDG Tech Devs site: http://darkmatter.ucsf.edu/
- Collaborative work http://mcmanuslab.ucsf.edu/
- NIH Reporter Link: Follow Link